pgas cis-reporter plasmids Search Results


prluc-c1  (BioSignal Group)
90
BioSignal Group prluc-c1
Prluc C1, supplied by BioSignal Group, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgas+cis-reporter+plasmids/pmc07091366-60-17-20?v=BioSignal+Group
Average 90 stars, based on 1 article reviews
prluc-c1 - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
renilla luciferase: prl-tk  (Promega)
90
Promega renilla luciferase: prl-tk
HEK293T cells were transfected with plasmids encoding different ZIKV proteins. Sixteen hours later, they were transfected with an NF‐κB promoter‐driven firefly <t>luciferase</t> plasmid (pNF‐κB‐luciferase) and a constitutively active <t>Renilla</t> luciferase construct (pRL‐TK), as well as 1 μg of poly(I:C). Twenty‐four hours later, cell lysates were harvested and subjected to luciferase assay. C, capsid; E, envelope. Values are expressed as mean ± standard error. *P < 0.05, **P < 0.01 (Student's t‐test); N = 3.
Renilla Luciferase: Prl Tk, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgas+cis-reporter+plasmids/pmc05283583-421-52-55?v=Promega
Average 90 stars, based on 1 article reviews
renilla luciferase: prl-tk - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier

Image Search Results


HEK293T cells were transfected with plasmids encoding different ZIKV proteins. Sixteen hours later, they were transfected with an NF‐κB promoter‐driven firefly luciferase plasmid (pNF‐κB‐luciferase) and a constitutively active Renilla luciferase construct (pRL‐TK), as well as 1 μg of poly(I:C). Twenty‐four hours later, cell lysates were harvested and subjected to luciferase assay. C, capsid; E, envelope. Values are expressed as mean ± standard error. *P < 0.05, **P < 0.01 (Student's t‐test); N = 3.

Journal: EMBO Reports

Article Title: Zika virus inhibits type‐I interferon production and downstream signaling

doi: 10.15252/embr.201642627

Figure Lengend Snippet: HEK293T cells were transfected with plasmids encoding different ZIKV proteins. Sixteen hours later, they were transfected with an NF‐κB promoter‐driven firefly luciferase plasmid (pNF‐κB‐luciferase) and a constitutively active Renilla luciferase construct (pRL‐TK), as well as 1 μg of poly(I:C). Twenty‐four hours later, cell lysates were harvested and subjected to luciferase assay. C, capsid; E, envelope. Values are expressed as mean ± standard error. *P < 0.05, **P < 0.01 (Student's t‐test); N = 3.

Article Snippet: Luciferase reporter assay A549 cells and HEK293T cells were transfected with the following promoter reporter (firefly luciferase) constructs IFIT1: pGL3B/561 (gift from Ganes Sen), IFN‐β: p125‐luc and IRF3: p55‐CIB‐Luc (provided by T. Taniguchi, University of Tokyo, Japan), NF‐κB: pNF‐kB‐Luc (Stratagene), ISRE: pGL4 ISRE (Promega), or GAS: pGAS‐Luc plasmid (Stratagene) along with the Renilla luciferase: pRL‐TK (Promega) as a transfection control.

Techniques: Transfection, Luciferase, Plasmid Preparation, Construct